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stvnbln
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Curriculum vitae

Steven Beelen

Your work is going to fill a large part of your life, and the only way to be truly satisfied is to do what you believe is great work. And the only way to do great work is to love what you do. If you haven’t found it yet, keep looking. Don’t settle. As with all matters of the heart, you’ll know when you find it.
— Steve Jobs
 
 

Education

College

2002-2007
Chemistry, Biochemistry
UCLL / KHLim - Katholieke Hogeschool Limburg, Campus B Diepenbeek
Professional Bachelor in chemistry, biochemistry • Graduaat • A1

 

Middle and High school

2000-2002
Chemistry (TSO)
VIA - St. Jozefsinstituut, Tienen
Chemie • A2

1998-2000
Techniek Wetenschappen (TSO)
VIA - St. Jozefsinstituut, Tienen

1996-1998
Moderne Talen - Wiskunde (ASO)
VIA - Immaculata Instituut, Tienen

 

 

Experience

jobs

2007-PRESENT
Research technician (Level 5.2 / HAY OZO Class 7)
at Lab for Biocrystallography (KULeuven, Dept of Pharmaceutical and Pharmacological Sciences)

Prof. S. Strelkov

Job description :

  • Scientific work for various projects (under supervision of Post-docs and Professor). Mainly cloning, protein production and purification and crystallization. Projects include :
    • Small Heat Shock Proteins
    • Virus Like Particles : BK Virus, JC Virus
    • Invertase (collaboration, crystallization)
    • PWWP (collaboration, crystallization)
  • Lab management : manager of the crystallization facility consisting of two Formulatrix Rock Imagers, a TTP Labtech Mosquito robot, a TTP Labtech Dragonfly robot, a Rigaku Micromax HF007 rotating anode X-ray source and Linux cluster ; manager of the liquid chromatography facility consisting of two FPLC systems (type Akta). Providing training, oversight and support (technical and scientific). 
  • General support : production and purification of in-house developed protease, writing and maintaining SOP for all scientific activities, maintenance of chemical stock, equipment and infrastructure, coordination of lab-activities during special events (visitation, construction, audits, ...).
  • HSE liaison for all HSE domains : developing and implementing safety policies, writing risk assessments, training new employees, lab-audit coordination, inventory.
  • Contact person for companies, students, visiting scholars, university staff.

›› Detailed job description used during the OFD assessment (in Dutch)
›› Example files : SOPs ; Digital lab notes ; Lab-safety training material
›› Publications : https://www.ncbi.nlm.nih.gov/pubmed/?term=strelkov+sv+beelen+s

 

2007
QC Lab technician (interim)
at Citrique Belge - DSM

Ir E. Decorte

Job description :

  • Quality analysis of samples taken from various steps during the production of citric acid. Checked parameters include purity (colorimetry), bead size (granulometry), acidity / quality (acid-base titration), water concentration (Karl-Fisher titration)
 

internships

2007 - 15 weeks
"Characterization of Colletotrichum isolates, cause of anthracnose on strawberries"
Proefcentrum Fruitteelt vzw, Gorsem
›› View my presentation and dissertation as presented on June 14, 2007

 

2002 - 3 weeks
"Determination of the control parameters of the citric acid production by Aspergillus niger"
Citrique Belge - DSM, Tienen
›› View my presentation as presented on June 21, 2002

 

 

Skills

SCIENTIFIC

Crystallography
Sitting and hanging drop, manual and automated using the TTP Labtech Mosquito nanoliter pipetting robot, data analysis using automated imaging robot (Formulatrix RockImager)

Analytical
Liquid chromatography using the GE Äkta system (IMAC, HIC, IEX, SEC)

Biochemistry
Expression and purification of fusion proteins in E.coli (His-SUMO tag , Arg-SUMO tag)

Molecular biology
SDS-PAGE, SLIC, Quick Change, DNA prep

 

EQUIPMENT

GE Äkta Pure / Purifier

  • Maintenance, administration, operation, training of new users
  • I created a set of pre-programmed methods that allows any user to easily control the Äkta system, from start to finish

Formulatrix RockImager

  • Maintenance, administration, operation, training of new users

TTP Labtech Mosquito

  • Maintenance, administration, operation, training of new users
  • I created a set of pre-programmed methods that allows any user to easily control the Mosquito system, from start to finish

Rigaku MicroMax 007HF

  • Maintenance, administration, operation, training of new users
 

KULeuven specific

HSE
Received training and certificates for all HSE domains (chemical safety, bio-safety, radiation protection, fire-safety and first emergency response)
Familiar with all procedures regarding chemical waste disposal, project declarations, permit request for hazardous chemicals

BIPP
Used and trained colleagues for the KULeuven inventory system and BIPP purchasing platform

 

it

General computer maintenance, troubleshooting and repair (updating OS, replacing components)

Software
MS Office (Word, Excel, Access) ; Akta Unicorn (programming, configuration, hardware maintenance) ; Apple Numbers and Keynote ; Formulatrix Rockmaker (programming, hardware maintenance) ; TTP Labtech Mosquito (programming, hardware maintenance)